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Tosoh Corporation
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VDS Optilab Chromatographietechnik
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Tosoh Corporation
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TriLink
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SAS institute
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Maisch GmbH
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BIOTAGE
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Image Search Results
Journal: Endocrinology
Article Title: Ghrelin, a Novel Growth Hormone-Releasing Acylated Peptide, Is Synthesized in a Distinct Endocrine Cell Type in the Gastrointestinal Tracts of Rats and Humans
doi: 10.1210/en.141.11.4255
Figure Lengend Snippet: FIG. 1. Representative RP-HPLC profiles of ghrelin immunoreactivity in rat stomach and jejunum. E, Data obtained using a RIA for ghrelin N-terminus; F, data obtained using a RIA for ghrelin C-terminus. The fundus (A; 5 mg) and jejunum (B; 125 mg) were chromatographed on a TSK ODS SIL 120A column (4.6 3 150 mm). A linear gradient of 10–60% CH3CN containing 0.1% TFA was run for 40 min at 1.0 ml/min. The fraction volume was 0.5 ml. Arrows indicate the elution positions of des-acyl rat ghrelin-(1–28) (a) and n-octanoylated rat ghrelin-(1–28) (b).
Article Snippet: The 125I-labeled peptides were purified on a
Techniques: